ATP:Mg²⁺ shapes material properties of protein-RNA condensates and their partitioning of clients.

ABSTRACT: Many cellular condensates are heterotypic mixtures of proteins and RNA formed in complex environments. Magnesium ions (Mg²⁺) and ATP can impact RNA folding, and local intracellular levels of these factors can vary significantly. However, the effect of ATP:Mg²⁺ on the material properties of protein-RNA condensates is largely unknown. Here, we use an in vitro condensate model of nucleoli, made from nucleophosmin 1 (NPM1) proteins and ribosomal RNA (rRNA), to study the effect of ATP:Mg²⁺. While NPM1 dynamics remain unchanged at increasing Mg²⁺ concentrations, the internal RNA dynamics dramatically slowed until a critical point, where gel-like states appeared, suggesting the RNA component alone forms a viscoelastic network that undergoes maturation d

SUBMITTER: Yewdall NA 

PROVIDER: S-EPMC9674983 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature