Multi-omics to predict changes during cold pressor test.

ABSTRACT:

Background

The cold pressor test (CPT) is a widely used pain provocation test to investigate both pain tolerance and cardiovascular responses. We hypothesize, that performing multi-omic analyses during CPT gives the opportunity to home in on molecular mechanisms involved. Twenty-two females were phenotypically assessed before and after a CPT, and blood samples were taken. RNA-Sequencing, steroid profiling and untargeted metabolomics were performed. Each 'omic level was analyzed separately at both single-feature and systems-level (principal component [PCA] and partial least squares [PLS] regression analysis) and all 'omic levels were combined using an integrative multi-omics approach, all using the paired-sample design.

Results

We showed that PCA was not able to discriminate

SUBMITTER: Kogelman LJA 

PROVIDER: S-EPMC9675059 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature