Escherichia coli harbouring strAB with reduced susceptibility towards gentamicin and amikacin: a single centre study from India.

ABSTRACT: In this study we report the presence of streptomycin resistance gene strAB within clinical isolates of Escherichia coli where streptomycin is not used to treat Gram-negative infections. In total, 135 E. coli isolates were obtained for the study. PCR based detection of strAB was performed in the study isolates followed by assessment of horizontal transferability. Cloning of strAB was done in laboratory strain E. coli DH5α. Pre-cloning and post-cloning susceptibility of the strain was done for assessment of acquired resistance. Among tested isolates, 89 were found to harbour strAB and it was encoded within a IncI1 type plasmid. Cloning experiments revealed the strAB gene showed unusual non-susceptibility towards amikacin and gentamicin.

SUBMITTER: Wangkheimayum J 

PROVIDER: S-EPMC9675172 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature