SrnaMapper: an optimal mapping tool for sRNA-Seq reads.

ABSTRACT:

Background

Sequencing is the key method to study the impact of short RNAs, which include micro RNAs, tRNA-derived RNAs, and piwi-interacting RNA, among others. The first step to make use of these reads is to map them to a genome. Existing mapping tools have been developed for long RNAs in mind, and, so far, no tool has been conceived for short RNAs. However, short RNAs have several distinctive features which make them different from messenger RNAs: they are shorter, they are often redundant, they can be produced by duplicated loci, and they may be edited at their ends.

Results

In this work, we present a new tool, srnaMapper, that exhaustively maps these reads with all these features in mind, and is most efficient when applied to reads no longer than 50 base pairs. We show, o

SUBMITTER: Zytnicki M 

PROVIDER: S-EPMC9675193 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature