Integrated T cell cytometry metrics for immune-monitoring applications in immunotherapy clinical trials.

ABSTRACT: Mass cytometry, or cytometry by TOF (CyTOF), provides a robust means of determining protein-level measurements of more than 40 markers simultaneously. While the functional states of immune cells occur along continuous phenotypic transitions, cytometric studies surveying cell phenotypes often rely on static metrics, such as discrete cell-type abundances, based on canonical markers and/or restrictive gating strategies. To overcome this limitation, we applied single-cell trajectory inference and nonnegative matrix factorization methods to CyTOF data to trace the dynamics of T cell states. In the setting of cancer immunotherapy, we showed that patient-specific summaries of continuous phenotypic shifts in T cells could be inferred from peripheral blood-derived CyTOF mass cytometry data. We furt

SUBMITTER: Sidiropoulos DN 

PROVIDER: S-EPMC9675468 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature