Serial Gene Expression Profiling of Neural Stem Cells Shows Transcriptome Switch by Long-Term Physioxia from Metabolic Adaption to Cell Signaling Profile.

ABSTRACT: Oxygen is an essential factor in the cellular microenvironment with pivotal effects on neural development with a particular sensitivity of midbrain neural stem cells (NSCs) to high atmospheric oxygen tension. However, most experiments are still performed at atmospheric O₂ levels (21%, normoxia), whereas mammalian brain tissue is physiologically exposed to substantially lower O₂ tensions around 3% (physioxia). We here performed serial Affymetrix gene array analyses to detect expression changes in mouse fetal NSCs from both midbrain and cortical tissues when kept at physioxia compared to normoxia. We identified more than 400 O₂-regulated genes involved in cellular metabolism, cell proliferation/differentiation, and various signaling pathways. NSCs from both r

SUBMITTER: Braunschweig L 

PROVIDER: S-EPMC9675612 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature