Peripheral blood mononuclear cell phenotype and function are maintained after overnight shipping of whole blood.

ABSTRACT: Same day processing of biospecimens such as blood is not always feasible, which presents a challenge for research programs seeking to study a broad population or to characterize patients with rare diseases. Recruiting sites may not be equipped to process blood samples and variability in timing and technique employed to isolate peripheral blood mononuclear cells (PBMCs) at local sites may compromise reproducibility across patients. One solution is to send whole blood collected by routine phlebotomy via overnight courier to the testing site under ambient conditions. Determining the impact of shipping on subsequent leukocyte responses is a necessary prerequisite to any experimental analysis derived from transported samples. To this end, whole blood was collected from healthy control subjects

SUBMITTER: Johnson RK 

PROVIDER: S-EPMC9675784 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature