Changes to the upper gastrointestinal microbiotas of children with reflux oesophagitis and oesophageal metaplasia.

ABSTRACT: Little is known of the relationships among paediatric upper gastrointestinal microbiotas, and the impact of medication use and disease on their diversity. Here, we investigated the diversity of three microbiotas in the upper gastrointestinal tract of paediatric patients in relation to each other and to host factors. Oral, oesophageal and gastric microbiotas from a prospective paediatric cohort (n=54) were profiled using the 16S rRNA gene and ITS2 amplicon sequencing. 16S rRNA gene amplicon sequencing of oesophageal biopsies from a retrospective paediatric cohort (n=96) and shotgun metagenomics data from oesophageal brushings (n=88) were employed for genomic signature validation. Bacterial diversity and composition showed substantial differences across oral, oesophageal

SUBMITTER: Luu LDW 

PROVIDER: S-EPMC9676027 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature