The Kleisin Subunits of Cohesin are Involved in the Fate Determination of Embryonic Stem Cells.

ABSTRACT: As a potential candidate to generate an everlasting cell source to treat various diseases, embryonic stem cells are regarded as a promising therapeutic tool in the regenerative medicine field. Cohesin, a multi-functional complex that controls various cellular activities, plays roles not only in organizing chromosome dynamics but also in controlling transcriptional activities related to self-renewal and differentiation of stem cells. Here, we report a novel role of the α-kleisin subunits of cohesin (RAD21 and REC8) in the maintenance of the balance between these two stem-cell processes. By knocking down REC8, RAD21, or the non-kleisin cohesin subunit SMC3 in mouse embryonic stem cells, we show that reduction in cohesin level impairs their self-renewal. Interestingly, the transcriptomic anal

SUBMITTER: Koh YE 

PROVIDER: S-EPMC9676991 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature