Comparison of monocytic cell lines U937 and THP-1 as macrophage models for in vitro studies.

ABSTRACT: Understanding macrophage biology can improve comprehension of diverse biological processes and provide insights into novel therapeutic immunomodulatory strategies. Due to limited yield and technical difficulty in isolating primary macrophages, in vitro studies commonly use monocytes as precursor cells. Monocytic cell lines are a virtually unlimited source of macrophage precursors and two of the most frequently used cell lines are THP-1 and U937. Besides a great variability in macrophage differentiation protocols there is scarce information on possible differences in the biological responses of these cell lines. In this study, we used a standardized differentiation protocol using PMA and compared the response of macrophages derived from THP-1 and U937 cells to M1-and M2-polarizing condition

SUBMITTER: Nascimento CR 

PROVIDER: S-EPMC9677084 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature