Endolysins of bacteriophage vB_Sal-S-S10 can naturally lyse Salmonella enteritidis.

ABSTRACT:

Background

The holin-endolysin lysis system plays an essential role in the phage life cycle. Endolysins are promising alternatives to antibiotics, and have been successfully used against Gram-positive bacteria. However, a few endolysins can externally lyse Gram-negative bacteria, due to the inaccessible peptidoglycan layer covered by the envelope.

Results

This study investigated the lysis system of a new Siphoviridae bacteriophage vB_Sal-S-S10 (S10), which, that was isolated from broiler farms, was found to be able to infect 51.4% (37/72) of tested S. enteritidis strains. Phage S10 genome had a classic holin-endolysin lysis system, except that one holin and one endolysin gene were functionally annotated. The orf 22 adjacent to the lysis cassette was identified as a new endol

SUBMITTER: Wang X 

PROVIDER: S-EPMC9677904 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature