Early Molecular Events Mediating Loss of Aquaporin-2 during Ureteral Obstruction in Rats.

ABSTRACT:

Background

Ureteral obstruction is marked by disappearance of the vasopressin-dependent water channel aquaporin-2 (AQP2) in the renal collecting duct and polyuria upon reversal. Most studies of unilateral ureteral obstruction (UUO) models have examined late time points, obscuring the early signals that trigger loss of AQP2.

Methods

We performed RNA-Seq on microdissected rat cortical collecting ducts (CCDs) to identify early signaling pathways after establishment of UUO.

Results

Vasopressin V2 receptor (AVPR2) mRNA was decreased 3 hours after UUO, identifying one cause of AQP2 loss. Collecting duct principal cell differentiation markers were lost, including many not regulated by vasopressin. Immediate early genes in CCDs were widely induced 3 hours after UUO, including

SUBMITTER: Sung CC 

PROVIDER: S-EPMC9678028 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature