Modeling single-cell phenotypes links yeast stress acclimation to transcriptional repression and pre-stress cellular states.

ABSTRACT: Stress defense and cell growth are inversely related in bulk culture analyses; however, these studies miss substantial cell-to-cell heterogeneity, thus obscuring true phenotypic relationships. Here, we devised a microfluidics system to characterize multiple phenotypes in single yeast cells over time before, during, and after salt stress. The system measured cell and colony size, growth rate, and cell-cycle phase along with nuclear trans-localization of two transcription factors: stress-activated Msn2 that regulates defense genes and Dot6 that represses ribosome biogenesis genes during an active stress response. By tracking cells dynamically, we discovered unexpected discordance between Msn2 and Dot6 behavior that revealed subpopulations of cells with distinct growth properties. Surprisingl

SUBMITTER: Bergen AC 

PROVIDER: S-EPMC9678356 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature