Anti-obesity effects of the dual-active adenosine A_2A/A₃ receptor-ligand LJ-4378.

ABSTRACT:

Background and objectives

A_2A adenosine receptor (A_2AAR)-mediated signaling in adipose tissues has been investigated as a potential target for obesity-related metabolic diseases. LJ-4378 has been developed as a dual-acting ligand with A_2AAR agonist and A₃ adenosine receptor (A₃AR) antagonist activity. The current study aimed to investigate the anti-obesity effects of LJ-4378 and its underlying molecular mechanisms.

Methods

Immortalized brown adipocytes were used for in vitro analysis. A high-fat diet (HFD)-induced obesity and cell death-inducing DFFA-like effector A reporter mouse models were used for in vivo experiments. The effects of LJ-4378 on lipolysis and mitochondrial metabolism were evaluated using immunoblotting

SUBMITTER: Kim K 

PROVIDER: S-EPMC9678795 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature