Assessing spermatozoal small ribonucleic acids and their relationship to blastocyst development in idiopathic infertile males.

ABSTRACT: Clinical testing strategies for diagnosing male factor infertility are limited. A deeper analysis of spermatozoa-derived factors could potentially diagnose some cases of 'unexplained infertility'. Spermatozoa carry a rich and dynamic profile of small RNAs, which have demonstrated potential developmental importance and association with fertility status. We used next-generation sequencing to correlate sperm small RNA profiles of normozoospermic males (n = 54) with differing blastocyst development rates, when using young donor oocytes. While ribosomal RNAs accounted for the highest number of sequencing reads, transfer RNA fragments of tRNA^Gly/GCC and tRNA^Val-CAC were the most abundant sequences across all sperm samples. A total of 324 small RNAs were differentially expre

SUBMITTER: Hamilton M 

PROVIDER: S-EPMC9678953 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature