Association between SEMA3A signaling pathway genes and BMD/OP risk: An epidemiological and experimental study.

ABSTRACT:

Objective

This study aimed to explore the associations of genetic variants in the semaphorin 3A (SEMA3A) signaling pathway genes, including SEMA3A, NRP1, PLXNA1, PLXNA2 and PLXNA3 with osteoporosis (OP) risk and bone mineral density (BMD) in a Chinese Han older adult population.

Study design and method

A two-stage design was adopted. Total of 47.8kb regions in the 5 genes were sequenced using targeted next-generation sequencing (NGS) technology in the discovery stage, and the discovered OP-related single nucleotide polymorphisms (SNPs) were further genotyped using improved multiple linkage detection reaction technique in the validation stage. Methods of ALP/TRAP staining, real-time fluorescent quantitative PCR, and cell proliferation and ap

SUBMITTER: Zhou HL 

PROVIDER: S-EPMC9679019 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature