Modeling Human Gonad Development in Organoids.

ABSTRACT:

Background

Our learning about human reproductive development is greatly hampered due to the absence of an adequate model. Animal studies cannot truthfully recapitulate human developmental processes, and studies of human fetal tissues are limited by their availability and ethical restrictions. Innovative three-dimensional (3D) organoid technology utilizing human pluripotent stem cells (hPSCs) offered a new approach to study tissue and organ development in vitro. However, a system for modeling human gonad development has not been established, thus, limiting our ability to study causes of infertility.

Methods

In our study we utilized the 3D hPSC organoid culture in mini-spin bioreactors. Relying on intrinsic self-organizing and differentiation capabilities of stem cells, we exp

SUBMITTER: Pryzhkova MV 

PROVIDER: S-EPMC9679106 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature