Comprehensive analysis of glycerolipid dynamics during tobacco pollen germination and pollen tube growth.

ABSTRACT: Pollen germination and subsequent pollen tube elongation are essential for successful land plant reproduction. These processes are achieved through well-documented activation of membrane trafficking and cell metabolism. Despite this, our knowledge of the dynamics of cellular phospholipids remains scarce. Here we present the turnover of the glycerolipid composition during the establishment of cell polarity and elongation processes in tobacco pollen and show the lipid composition of pollen plasma membrane-enriched fraction for the first time. To achieve this, we have combined several techniques, such as lipidomics, plasma membrane isolation, and live-cell microscopy, and performed a study with different time points during the pollen germination and pollen tube growth. Our results showed that

SUBMITTER: Serrano N 

PROVIDER: S-EPMC9679300 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature