RNA profiling of circulating tumor cells systemically captured from diagnostic leukapheresis products in prostate cancer patients.

ABSTRACT: The application of circulating tumor cells (CTCs) in both clinical practice and research has been continuously limited by the rare number of targets that can be found in a tube of peripheral blood. Diagnostic leukapheresis (DLA) was used to increase the sampling volume. AdnaTest was used to process the whole leukopak, and the RNAs of captured CTCs was then profiled by NanoString nCounter platform. Spike-in experiments and leukopaks from patients with metastatic prostate cancer were used to validate this new strategy. The whole leukopak was further concentrated five times to reduce the total volume from 150 ​mL to 30 ​mL, which enabled it to be processed by 3 separate AdnaTest kits. The spike-in experiment demonstrated a reliable capture when there were more than 100 cancer cells/10 ​mL of

SUBMITTER: Dong L 

PROVIDER: S-EPMC9679687 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature