Engineering a Rhodopsin-Based Photo-Electrosynthetic System in Bacteria for CO₂ Fixation.

ABSTRACT: A key goal of synthetic biology is to engineer organisms that can use solar energy to convert CO₂ to biomass, chemicals, and fuels. We engineered a light-dependent electron transfer chain by integrating rhodopsin and an electron donor to form a closed redox loop, which drives rhodopsin-dependent CO₂ fixation. A light-driven proton pump comprising Gloeobacter rhodopsin (GR) and its cofactor retinal have been assembled in Ralstonia eutropha (Cupriavidus necator) H16. In the presence of light, this strain fixed inorganic carbon (or bicarbonate) leading to 20% growth enhancement, when formate was used as an electron donor. We found that an electrode from a solar panel can replace organic compounds to serve as the electron donor, mediated by the electr

SUBMITTER: Davison PA 

PROVIDER: S-EPMC9680020 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature