Studies on HBcAg-rBlo t 5-21 Fusion Protein Vaccine That Alleviates Blomia tropicalis Airway Inflammation.

ABSTRACT:

Background

In tropical and subtropical areas, allergens from the dust mite species Blomia tropicalis are common causes of allergic rhinitis and asthma. Blomia tropicalis has two main allergens: Blo t 5 and Blo t 21.

Aim

To generate a chimeric virus-like particle containing HBcAg, Blo t 5 and Blo t 21 that can treat allergies caused by Blomia tropicalis.

Methods

To produce allergic asthma in mice, prokaryotic expression and purification of Blomia tropicalis allergens rBlo t 5, rBlo t 21, and recombinant fusion allergen rBlo t 5-21 were utilized in the study. We created a hepatitis B core antigen (HBcAg) and rBlo t 5-21 fusion prokaryotic expression plasmid. HBcAg-rBlo t 5-21 was purified after expression and tested by transmission electron m

SUBMITTER: Pei Y 

PROVIDER: S-EPMC9680538 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature