Increasing the Thermodynamic Driving Force of the Phosphofructokinase Reaction in Clostridium thermocellum.

ABSTRACT: Glycolysis is an ancient, widespread, and highly conserved metabolic pathway that converts glucose into pyruvate. In the canonical pathway, the phosphofructokinase (PFK) reaction plays an important role in controlling flux through the pathway. Clostridium thermocellum has an atypical glycolysis and uses pyrophosphate (PP_i) instead of ATP as the phosphate donor for the PFK reaction. The reduced thermodynamic driving force of the PP_i-PFK reaction shifts the entire pathway closer to thermodynamic equilibrium, which has been predicted to limit product titers. Here, we replace the PP_i-PFK reaction with an ATP-PFK reaction. We demonstrate that the local changes are consistent with thermodynamic predictions: the ratio of fructose 1,6-bisphosphate to fructose-6-pho

SUBMITTER: Hon S 

PROVIDER: S-EPMC9680637 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature