Fibrin prestress due to platelet aggregation and contraction increases clot stiffness.

ABSTRACT: Efficient hemorrhagic control is attained through the formation of strong and stable blood clots at the site of injury. Although it is known that platelet-driven contraction can dramatically influence clot stiffness, the underlying mechanisms by which platelets assist fibrin in resisting external loads are not understood. In this study, we delineate the contribution of platelet-fibrin interactions to clot tensile mechanics using a combination of new mechanical measurements, image analysis, and structural mechanics simulation. Based on uniaxial tensile test data using custom-made microtensometer and fluorescence microscopy of platelet aggregation and platelet-fibrin interactions, we show that integrin-mediated platelet aggregation and actomyosin-driven platelet contraction synergistically i

SUBMITTER: Pathare SJ 

PROVIDER: S-EPMC9680775 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature