Cell cycle-dependent endocytosis of DNA-wrapped single-walled carbon nanotubes by neural progenitor cells.

ABSTRACT: While exposure of C17.2 neural progenitor cells (NPCs) to nanomolar concentrations of carbon nanotubes (NTs) yields evidence of cellular substructure reorganization and alteration of cell division and differentiation, the mechanisms of NT entry are not understood. This study examines the entry modes of (GT)₂₀ DNA-wrapped single-walled carbon nanotubes (SWCNTs) into NPCs. Several endocytic mechanisms were examined for responsibility in nanomaterial uptake and connections to alterations in cell development via cell-cycle regulation. Chemical cell-cycle arrest agents were used to synchronize NPCs in early G₁, late G₁/S, and G₂/M phases at rates (>80%) aligned with previously documented levels of synchrony for stem cells. Synchronization led to the h

SUBMITTER: Chandrasekar S 

PROVIDER: S-EPMC9680777 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature