Advanced quantification for single-cell adhesion by variable-angle TIRF nanoscopy.

ABSTRACT: Over the last decades, several techniques have been developed to study cell adhesion; however, they present significant shortcomings. Such techniques mostly focus on strong adhesion related to specific protein-protein associations, such as ligand-receptor binding in focal adhesions. Therefore, weak adhesion, related to less specific or nonspecific cell-substrate interactions, are rarely addressed. Hence, we propose in this work a complete investigation of cell adhesion, from highly specific to nonspecific adhesiveness, using variable-angle total internal reflection fluorescence (vaTIRF) nanoscopy. This technique allows us to map in real time cell topography with a nanometric axial resolution, along with cell cortex refractive index. These two key parameters allow us to distinguish high and

SUBMITTER: El Arawi D 

PROVIDER: S-EPMC9680782 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature