Fine-tuning of FeS proteins monitored via pulsed EPR redox potentiometry at Q-band.

ABSTRACT: As essential electron translocating proteins in photosynthetic organisms, multiple plant-type ferredoxin (Fdx) isoforms are involved in a high number of reductive metabolic processes in the chloroplast. To allow quick cellular responses under changing environmental conditions, different plant-type Fdxs in Chlamydomonas reinhardtii were suggested to have adapted their midpoint potentials to a wide range of interaction partners. We performed pulsed electron paramagnetic resonance (EPR) monitored redox potentiometry at Q-band on three Fdx isoforms for a straightforward determination of their midpoint potentials. Additionally, site-directed mutagenesis was used to tune the midpoint potential of CrFdx1 in a range of approximately -338 to -511 mV, confirming the importance of singl

SUBMITTER: Heghmanns M 

PROVIDER: S-EPMC9680799 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature