Rapid ensemble measurement of protein diffusion and probe blinking dynamics in cells.

ABSTRACT: We present a fluorescence fluctuation image correlation analysis method that can rapidly and simultaneously measure the diffusion coefficient, photoblinking rates, and fraction of diffusing particles of fluorescent molecules in cells. Unlike other image correlation techniques, we demonstrated that our method could be applied irrespective of a nonuniformly distributed, immobile blinking fluorophore population. This allows us to measure blinking and transport dynamics in complex cell morphologies, a benefit for a range of super-resolution fluorescence imaging approaches that rely on probe emission blinking. Furthermore, we showed that our technique could be applied without directly accounting for photobleaching. We successfully employed our technique on several simulations with realistic EMC

SUBMITTER: Sehayek S 

PROVIDER: S-EPMC9680803 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature