Aspirin Mediates Its Antitumoral Effect Through Inhibiting PTTG1 in Pituitary Adenoma.

ABSTRACT:

Context

DNA demethylation and inhibitory effects of aspirin on pituitary cell proliferation have been demonstrated.

Objective

Our aim was to clarify the molecular mechanisms behind the aspirin-related effects in pituitary cells.

Methods

DNA methylome and whole transcriptome profile were investigated in RC-4B/C and GH3 pituitary cell lines upon aspirin treatment. Effects of aspirin and a demethylation agent, decitabine, were further tested in vitro. PTTG1 expression in 41 human PitNET samples and whole genome gene and protein expression data of 76 PitNET and 34 control samples (available in Gene Expression Omnibus) were evaluated.

Results

Aspirin induced global DNA demethylation and consequential transcriptome changes. Overexpression of Tet enzymes and their cof

SUBMITTER: Szabo B 

PROVIDER: S-EPMC9681612 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature