Signal sequence contributes to the immunogenicity of Pasteurella multocida lipoprotein E.

ABSTRACT: Recombinant Pasterurella multocida lipoprotein E (PlpE) has been shown to protect against fowl cholera. This study aimed to determine if the signal sequence may contribute to the antigenicity and protective efficacy of recombinant PlpE. A small antigenic domain of PlpE (termed truncated PlpE, tPlpE) was constructed with (SP-tPlpE) or without (tPlpE) the signal sequence and evaluated in vitro and in vivo. In vitro, the HEK-Bule hTLR2 Cells were used to evaluate the activation of NF-kB in the test associated with the stimulation of the SP-tPlpE and tPlpE proteins. When chickens were immunized, compared to the tPlpE vaccine group, the SP-tPlpE group showed higher antibody levels and enhanced CD4⁺ T cell response. In a challenge test, the SP-tPlpE group showed a survival rate of 87.

SUBMITTER: Cheng LT 

PROVIDER: S-EPMC9681653 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature