PET/MR Imaging of a Lung Metastasis Model of Clear Cell Renal Cell Carcinoma with (2S,4R)-4-[¹⁸F]Fluoroglutamine.

ABSTRACT:

Purpose

Metabolic reprogramming plays an important role in the tumorigenesis of clear cell renal cell carcinoma (ccRCC). Currently, positron emission tomography (PET) reporters are not used clinically to visualize altered glutamine metabolism in ccRCC, which greatly hinders detection, staging, and real-time therapeutic assessment. We sought to determine if (2S,4R)-4-[¹⁸F]fluoroglutamine ([¹⁸F]FGln) could be used to interrogate altered glutamine metabolism in ccRCC lesions in the lung.

Procedures

We generated a novel ccRCC lung lesion model using the ccRCC cell line UMRC3 stably transfected with GFP and luciferase constructs. This cell line was used for characterization of [¹⁸F]FGln uptake and retention by transport analysis in cell culture an

SUBMITTER: Pollard AC 

PROVIDER: S-EPMC9681699 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature