A CRISPR endonuclease gene drive reveals distinct mechanisms of inheritance bias.

ABSTRACT: CRISPR/Cas gene drives can bias transgene inheritance through different mechanisms. Homing drives are designed to replace a wild-type allele with a copy of a drive element on the homologous chromosome. In Aedes aegypti, the sex-determining locus is closely linked to the white gene, which was previously used as a target for a homing drive element (w^GDe). Here, through an analysis using this linkage we show that in males inheritance bias of w^GDe did not occur by homing, rather through increased propagation of the donor drive element. We test the same w^GDe drive element with transgenes expressing Cas9 with germline regulatory elements sds3, bgcn, and nup50. We only find inheritance bias through homing, even with the identical nup50-Cas9 transgene. We propose t

SUBMITTER: Verkuijl SAN 

PROVIDER: S-EPMC9681865 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature