PHLIP Peptides Target Acidity in Activated Macrophages.

ABSTRACT:

Purpose

Acidity can be a useful alternative biomarker for the targeting of metabolically active cells in certain diseased tissues, as in acute inflammation or aggressive tumors. We investigated the targeting of activated macrophages by pH low insertion peptides (pHLIPs), an established technology for targeting cell-surface acidity.

Procedures

The uptake of fluorescent pHLIPs by activated macrophages was studied in cell cultures, in a mouse model of lung inflammation, and in a mouse tumor model. Fluorescence microscopy, whole-body and organ imaging, immunohistochemistry, and FACS analysis were employed.

Results

We find that cultured, activated macrophages readily internalize pHLIPs. The uptake is higher in glycolytic macrophages activated by LPS and INF-γ compared to m

SUBMITTER: Visca H 

PROVIDER: S-EPMC9681937 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature