Follicular fluid steroid and gonadotropic hormone levels and mitochondrial function from exosomes predict embryonic development.

ABSTRACT:

Purpose

Human follicular fluid (FF) is a complex biological fluid that contributes to the micro-environment of oocyte development. The aim of this study was to evaluate the role of steroid and gonadotropic hormones levels and mitochondrial function in embryo development during in vitro fertilization cycles.

Methods

This was a cohort study of 138 women receiving IVF/ICSI, including 136 FF samples from 109 infertile women. FF steroid and gonadotropic hormones levels were tested by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and immunoassays. The mRNA expression levels of mitochondrial electron transport chain (ETC) complex genes from FF exosomes were detected by qPCR.

Results

Analysis of these individual FF concentrations revealed that LH and FSH co

SUBMITTER: Yu L 

PROVIDER: S-EPMC9682035 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature