Cloning and Expression of a New Trichinella spiralis Serine Protease and Its Role in Invading Host Intestinal Epithelium.

ABSTRACT:

Background

In previous studies, a new Trichinella spiralis serine protease 1.1 (TsSP1.1) was identified in surface proteins of T. spiralis muscle larvae (ML) by proteomics analysis, but its functions in T. spiralis infection are unknown. The aim of this study was to investigate the roles of TsSP1.1 during larval intrusion of gut epithelium.

Methods

From January 2019 to March 2021, complete TsSP1.1 cDNA sequence was cloned and expressed in Escherichia coli BL21 at the Department of Parasitology, Medical College of Zhengzhou University, Zhengzhou, China. Expression and location of TsSP1.1 in the parasite were investigated using indirect immunofluorescence assay (IIFA) and Western blotting. The in vitro intestinal epithelium cells (IECs) intru

SUBMITTER: Liu F 

PROVIDER: S-EPMC9682372 | biostudies-literature | 2022 Jul-Sep

REPOSITORIES: biostudies-literature