Toward Realistic Dosimetry In Vitro: Determining Effective Concentrations of Test Substances in Cell Culture and Their Prediction by an In Silico Mass Balance Model.

ABSTRACT: Nominal concentrations (C_Nom) in cell culture media are routinely used to define concentration-effect relationships in the in vitro toxicology. The actual concentration in the medium (C_Medium) can be affected by adsorption processes, evaporation, or degradation of chemicals. Therefore, we measured the total and free concentration of 12 chemicals, covering a wide range of lipophilicity (log K_OW -0.07-6.84), in the culture medium (C_Medium) and cells (C_Cell) after incubation with Balb/c 3T3 cells for up to 48 h. Measured values were compared to predictions using an as yet unpublished in silico mass balance model that combined relevant equations from similar models published by others. The t

SUBMITTER: Dimitrijevic D 

PROVIDER: S-EPMC9682521 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature