Downregulation of SEPTIN5 inhibits prostate cancer progression by increasing CD8⁺ T cell infiltration.

ABSTRACT: Background: Prostate cancer (PCa) is one of the most common carcinomas in men, and aberrant expression of SEPTIN5 (SEPT5) has been detected in PCa tissues. However, the role of SEPT5 in PCa is still unclear. In this study, we attempted to explore the expression changes, clinical relevance and immunomodulatory function of SEPT5 in PCa. Methods: The expression and clinical significance of SEPT5 were evaluated based on bioinformatic analysis and were verified by western blotting, real time PCR and IHC. Allograft mouse models were used to assess the role of SEPT5 on PCa tumour formation and immunomodulatory function. Mass cytometry and IHC were used to determine the effects of SEPT5 on immune cell infiltration, especially CD8⁺ T cell infiltration. Correlations between

SUBMITTER: Chang M 

PROVIDER: S-EPMC9682533 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature