Development of a novel defined minimal medium for Gluconobacter oxydans 621H by systematic investigation of metabolic demands.

ABSTRACT:

Background

Historically, complex media are used for the cultivation of Gluconobacter oxydans in industry and research. Using complex media has different drawbacks like higher costs for downstream processing and significant variations in fermentation performances. Synthetic media can overcome those drawbacks, lead to reproducible fermentation performances. However, the development of a synthetic medium is time and labour consuming. Detailed knowledge about auxotrophies and metabolic requirements of G. oxydans is necessary. In this work, we use a systematic approach applying the in-house developed μRAMOS technology to identify auxotrophies and develop a defined minimal medium for cultivation of G. oxydans fdh, improving the production process of the natural sweetener 5-ketofructose.<

SUBMITTER: Battling S 

PROVIDER: S-EPMC9682679 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature