Modification of the Folmer primers for the cytochrome c oxidase gene facilitates identification of mosquitoes.

ABSTRACT:

Background

Accurate identification of mosquito species is essential for the development and optimization of strategies to control mosquitoes and mosquito-borne diseases. Problems with the morphological identification of mosquito species have led to the use of molecular identification techniques, in particular the Folmer cytochrome c oxidase subunit I (COI) PCR system (FCOS), originally designed to identify a range of other invertebrates.

Methods

As there can be difficulties identifying mosquitoes using FCOS, we re-evaluated the FCOS primers and developed a new COI-based SYBR PCR (the Auburn COI system-AUCOS) to improve the molecular identification of mosquitoes. Sequence data in GenBank for 33 species from 10 genera of mosquitoes were used to develop our AUCOS primers. Two m

SUBMITTER: Hoque MM 

PROVIDER: S-EPMC9682724 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature