ALKBH5 in mouse testicular Sertoli cells regulates Cdh2 mRNA translation to maintain blood-testis barrier integrity.

ABSTRACT:

Background

RNA N⁶-methyladenosine (m⁶A) is involved in mammalian spermatogenesis. In both germ cells and Leydig cells, ALKBH5 regulates spermatogenesis and androgen synthesis in an m⁶A-dependent manner. However, it is unclear whether ALKBH5 plays a role in testicular Sertoli cells, which constitute the blood-testis barrier (BTB) through cell junctions between adjacent Sertoli cells.

Methods

ALKBH5 expression in the testes of humans and mice was detected by immunohistochemical staining and immunofluorescence staining. BTB integrity was evaluated by BTB assay. m⁶A-seq was performed to screen for BTB-related molecules regulated by ALKBH5. m⁶A immunoprecipitation-quantitative real-time polymerase chain reaction (qPCR), RNA i

SUBMITTER: Cai Z 

PROVIDER: S-EPMC9682758 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature