Primary neurons lacking the SNAREs vti1a and vti1b show altered neuronal development.

ABSTRACT:

Background

Neurons are highly specialized cells with a complex morphology generated by various membrane trafficking steps. They contain Golgi outposts in dendrites, which are formed from somatic Golgi tubules. In trafficking membrane fusion is mediated by a specific combination of SNARE proteins. A functional SNARE complex contains four different helices, one from each SNARE subfamily (R-, Qa, Qb and Qc). Loss of the two Qb SNAREs vti1a and vti1b from the Golgi apparatus and endosomes leads to death at birth in mice with massive neurodegeneration in peripheral ganglia and defective axon tracts.

Methods

Hippocampal and cortical neurons were isolated from Vti1a^-/- Vti1b^-/- double deficient, Vti1a^-/- Vti1b^+/-, Vti1a^+/- Vti1

SUBMITTER: Bollmann C 

PROVIDER: S-EPMC9682837 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature