WTAP Targets the METTL3 m⁶A-Methyltransferase Complex to Cytoplasmic Hepatitis C Virus RNA to Regulate Infection.

ABSTRACT: Modification of the hepatitis C virus (HCV) positive-strand RNA genome by N6-methyladenosine (m⁶A) regulates the viral life cycle. This life cycle takes place solely in the cytoplasm, while m⁶A addition on cellular mRNA takes place in the nucleus. Thus, the mechanisms by which m⁶A is deposited on the viral RNA have been unclear. In this work, we find that m⁶A modification of HCV RNA by the m⁶A-methyltransferase proteins methyltransferase-like 3 and 14 (METTL3 and METTL14) is regulated by Wilms' tumor 1-associating protein (WTAP). WTAP, a predominantly nuclear protein, is an essential member of the cellular mRNA m⁶A-methyltransferase complex and known to target METTL3 to mRNA. We found that HCV infection induces localization

SUBMITTER: Sacco MT 

PROVIDER: S-EPMC9683008 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature