Cells Adapt to Resist Fluoride through Metabolic Deactivation and Intracellular Acidification.

ABSTRACT: Fluoride is highly abundant in the environment. Many organisms have adapted specific defense mechanisms against high concentrations of fluoride, including the expression of proteins capable of removing fluoride from cells. However, these fluoride transporters have not been identified in all organisms, and even organisms that express fluoride transporters vary in tolerance capabilities across species, individuals, and even tissue types. This suggests that alternative factors influence fluoride tolerance. We screened for adaptation against fluoride toxicity through an unbiased mutagenesis assay conducted on Saccharomyces cerevisiae lacking the fluoride exporter FEX, the primary mechanism of fluoride resistance. Over 80 independent fluoride-hardened strains were generated, with anywher

SUBMITTER: Johnston NR 

PROVIDER: S-EPMC9683101 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature