Three types of Leishmania mexicana amastigotes: Proteome comparison by quantitative proteomic analysis.

ABSTRACT: Leishmania is the unicellular parasite transmitted by phlebotomine sand fly bite. It exists in two different forms; extracellular promastigotes, occurring in the gut of sand flies, and intracellular, round-shaped amastigotes residing mainly in vertebrate macrophages. As amastigotes originating from infected animals are often present in insufficient quality and quantity, two alternative types of amastigotes were introduced for laboratory experiments: axenic amastigotes and amastigotes from macrophages infected in vitro. Nevertheless, there is very little information about the degree of similarity/difference among these three types of amastigotes on proteomic level, whose comparison is crucial for assessing the suitability of using alternative types of amastigotes in experiment

SUBMITTER: Pacakova L 

PROVIDER: S-EPMC9683477 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature