A low-cost, open-source evolutionary bioreactor and its educational use.
Ontology highlight
ABSTRACT:
SUBMITTER: Gopalakrishnan V
PROVIDER: S-EPMC9683782 | biostudies-literature | 2022 Nov
REPOSITORIES: biostudies-literature
Publications
A low-cost, open-source evolutionary bioreactor and its educational use.
Gopalakrishnan Vishhvaan V Crozier Dena D Card Kyle J KJ Chick Lacy D LD Krishnan Nikhil P NP McClure Erin E Pelesko Julia J Williamson Drew F K DFK Nichol Daniel D Mandal Soumyajit S Bonomo Robert A RA Scott Jacob G JG
eLife 20221101
A morbidostat is a bioreactor that uses antibiotics to control the growth of bacteria, making it well-suited for studying the evolution of antibiotic resistance. However, morbidostats are often too expensive to be used in educational settings. Here we present a low-cost morbidostat called the EVolutionary biorEactor (EVE) that can be built by students with minimal engineering and programming experience. We describe how we validated EVE in a real classroom setting by evolving replicate <i>Escheri ...[more]
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