Quantifying fluorescent nanoparticle uptake in mammalian cells using a plate reader.

ABSTRACT: In keeping with the rapid expansion of nanoparticle applications, various tools are required to investigate how nanoparticles interact with biological entities. Many assays have been developed to measure the cellular uptake of nanoparticles, but so far most of the methods are laborious and often non-quantitative. Here we developed an easily accessible and robust quantitative measurement method of the level of cellular uptake of fluorescently labeled nanoparticles using a plate reader. In the experimental design, potential issues that could lead to measurement variation were identified and addressed. For example, the variation in fluorescence intensity of samples due to differences in cell number was normalized to optical density, which is a physical value corresponding to the cell number.

SUBMITTER: Shin HJ 

PROVIDER: S-EPMC9684140 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature