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Biotechnologically produced chitosans with nonrandom acetylation patterns differ from conventional chitosans in properties and activities.

ABSTRACT: Chitosans are versatile biopolymers with multiple biological activities and potential applications. They are linear copolymers of glucosamine and N-acetylglucosamine defined by their degree of polymerisation (DP), fraction of acetylation (F_A), and pattern of acetylation (PA). Technical chitosans produced chemically from chitin possess defined DP and F_A but random PA, while enzymatically produced natural chitosans probably have non-random PA. This natural process has not been replicated using biotechnology because chitin de-N-acetylases do not efficiently deacetylate crystalline chitin. Here, we show that such enzymes can partially N-acetylate fully deacetylated chitosan in the presence of excess acetate, yielding chitosans with F_A up to 0.7 and an enzyme-dependent non-random PA. The biotech chitosans differ from technical chitosans both in terms of physicochemical and nanoscale solution properties and biological activities. As with synthetic block co-polymers, controlling the distribution of building blocks within the biopolymer chain will open a new dimension of chitosan research and exploitation.

SUBMITTER: Sreekumar S

PROVIDER: S-EPMC9684148 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature

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Biotechnologically produced chitosans with nonrandom acetylation patterns differ from conventional chitosans in properties and activities.

Sreekumar Sruthi S Wattjes Jasper J Niehues Anna A Mengoni Tamara T Mendes Ana C AC Morris Edwin R ER Goycoolea Francisco M FM Moerschbacher Bruno M BM

Nature communications 20221121 1

Chitosans are versatile biopolymers with multiple biological activities and potential applications. They are linear copolymers of glucosamine and N-acetylglucosamine defined by their degree of polymerisation (DP), fraction of acetylation (F<sub>A</sub>), and pattern of acetylation (PA). Technical chitosans produced chemically from chitin possess defined DP and F<sub>A</sub> but random PA, while enzymatically produced natural chitosans probably have non-random PA. This natural process has not bee ...[more]

PMID: 36418307

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Biotechnologically produced chitosans with nonrandom acetylation patterns differ from conventional chitosans in properties and activities.

Publications

Biotechnologically produced chitosans with nonrandom acetylation patterns differ from conventional chitosans in properties and activities.

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