The genetic and biochemical determinants of mRNA degradation rates in mammals.

ABSTRACT:

Background

Degradation rate is a fundamental aspect of mRNA metabolism, and the factors governing it remain poorly characterized. Understanding the genetic and biochemical determinants of mRNA half-life would enable more precise identification of variants that perturb gene expression through post-transcriptional gene regulatory mechanisms.

Results

We establish a compendium of 39 human and 27 mouse transcriptome-wide mRNA decay rate datasets. A meta-analysis of these data identified a prevalence of technical noise and measurement bias, induced partially by the underlying experimental strategy. Correcting for these biases allowed us to derive more precise, consensus measurements of half-life which exhibit enhanced consistency between species. We trained substantially improved

SUBMITTER: Agarwal V 

PROVIDER: S-EPMC9684954 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature