Colorimetric and Raman dual-mode lateral flow immunoassay detection of SARS-CoV-2 N protein antibody based on Ag nanoparticles with ultrathin Au shell assembled onto Fe₃O₄ nanoparticles.

ABSTRACT: Serological antibody tests are useful complements of nuclei acid detection for SARS-CoV-2 diagnosis, which can significantly improve diagnostic accuracy. However, antibody detection in serum or plasma remains challenging to do with high sensitivity. In this study, Ag nanoparticles with ultra-thin Au shells embedded with 4-mercaptobenzoic acid (MBA) (Ag^MBA@Au) were manufactured and then assembled onto Fe₃O₄ surface by electrostatic interaction to construct the Fe₃O₄-Ag^MBA@Au nanoparticles (NPs) with magnetic-Raman-colorimetric properties. Based on the composite nanoparticles, a colorimetric and Raman dual-mode lateral flow immunoassay (LFIA) for ultrasensitive identification of SARS-CoV-2 nucleocapsid (N) protein antibody was

SUBMITTER: Li J 

PROVIDER: S-EPMC9685096 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature