Stability and conformational memory of electrosprayed and rehydrated bacteriophage MS2 virus coat proteins.

ABSTRACT: Proteins are innately dynamic, which is important for their functions, but which also poses significant challenges when studying their structures. Gas-phase techniques can utilise separation and a range of sample manipulations to transcend some of the limitations of conventional techniques for structural biology in crystalline or solution phase, and isolate different states for separate interrogation. However, the transfer from solution to the gas phase risks affecting the structures, and it is unclear to what extent different conformations remain distinct in the gas phase, and if resolution in silico can recover the native conformations and their differences. Here, we use extensive molecular dynamics simulations to study the two distinct conformations of dimeric capsid protein of t

SUBMITTER: Brodmerkel MN 

PROVIDER: S-EPMC9685359 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature