Downregulation of SOCS1 increases interferon-induced ISGylation during differentiation of induced-pluripotent stem cells to hepatocytes.

ABSTRACT:

Background & aims

Increased expression of IFN-stimulated gene 15 (ISG15) and subsequently increased ISGylation are key factors in the host response to viral infection. In this study, we sought to characterize the expression of ISG15, ISGylation, and associated enzymes at each stage of differentiation from induced pluripotent stem cells (iPSCs) to hepatocytes.

Methods

To study the regulation of ISGylation, we utilized patient samples and in vitro cell culture models including iPSCs, hepatocytes-like cells, immortalized cell lines, and primary human hepatocytes. Protein/mRNA expression were measured following treatment with poly(I:C), IFNα and HCV infection.

Results

When compared to HLCs, we observed several novel aspects of the ISGylation pathway in iPSCs. These

SUBMITTER: Edwards JS 

PROVIDER: S-EPMC9685392 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature